![]() WebI want to calculate EC50 of these curves in GraphPad prism. How to report standard deviation for IC50 calculation? ![]() Prism includes an equationthat fits any EC value directly. ![]() With such experiments, … See more A simple rearrangement of the equation lets you fit EC80 (or EC90 or ECanything) instead of the EC50. With more inhibitor, the response decreases, so the dose-response curve goes downhill. There is no particular advantage … See more In many experiments, you vary the concentration of an inhibitor. If the EC50 equals 1 micromolar (10-6molar), the log EC50 is –6 and the pEC50 is 6. Depending on how you have normalized … See more The pEC50 is defined as the negative logarithm of the EC50. It is impossible to define the EC50 until you first define the baseline and maximum response. ![]() The EC50 is defined quite simply as the concentration of agonist that provokes a response halfway between the baseline (Bottom) and maximum response (Top). GraphPad Prism 7 Curve Fitting Guide - The EC50 ![]()
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